| 1101 |
Immune-enhancing effects of Echinacea purpurea extracts on RAW264.7 cells via TLR4-mediated NF-κB and MAPKs pathways |
Hae-Lim Kim,Sung-Kwon Lee, Bong-Keun Choi, Dong-Ryung Lee |
20231025 ~ |
학회지 |
| Echinacea purpurea (L.) Moench is a medicinal plant from North America, Europe, and Australia that has been traditionally used to treat the common cold, bronchitis, coughs, and inflammation of the pharynx and mouth. Furthermore, extracts of Echinacea purpurea (EP) exert various biological effects, such as antioxidant, antibacterial, and anti-inflammatory activities. However, the possible mechanisms of action of the immune-enhancing effects are yet to be elucidated. Therefore, this study investigated the role of EP extracts in the immune-enhancing effects of RAW264.7 cells and the underlying mechanisms of action. It was found that EP extracts considerably increased the protein expression of iNOS, COX-2, and mPGES-1 in RAW264.7 cells. Also, EP extracts increased NO production, phagocytic activity, and the expression of cytokines. Consistent with these results, phosphorylation of MAPKs (ERK, JNK, and p38) and NF-κB (IKKα/β, IκBα, and NF-κB p65) were induced after treatment with EP extracts. Finally, EP extracts caused a marked increase in activation of the TLR4-TRAF6-TAK1 pathway. These results suggest that the immune-enhancing effects of EP extracts are mediated through the TLR4-associated activation of the NF-κB and MAPK pathways in RAW264.7 cells. Thus, it is suggested that EP extracts could be considered as a potential immunostimulatory agent or functional food. |
| 1100 |
멜라토닌 대사를 바탕으로 본 황제내경 소문 사기조신대론 제1장 |
박신영,류호룡, 조정효, 최정은, 박상수, 박미소 |
20230825 ~ |
학회지 |
| This paper aims to reinterpret an ancient Chinese medical book called "The Yellow Emperor's Classic of Medicine" (Hwangjenaegyeong in Korean) from the perspective of melatonin and antioxidant action. In the main body of the paper, we will first explain the changes in circadian rhythms according to the seasons through the first chapter of Somun "Sagijosindaelon". Spring, summer, autumn, and winter are introduced, and their relevance to disease is discussed. Next, the role of the suprachiasmatic nucleus in regulating circadian rhythms in mammals and the synchronization of peripheral clocks with seasonal changes is described. The relationship between light stimulation and melatonin synthesis is then addressed, noting that melatonin synthesis is normally inhibited in the presence of light and produced in the dark. However, recent studies have shown that there are more forms of melatonin that are produced in the presence of sunlight. Finally, it emphasizes the effect of light exposure on the biological clock and the importance of melatonin produced in the mitochondria, suggesting that we should focus on increasing melatonin levels. This paper reinterprets "The Yellow Emperor's Classic of Medicine" from the perspective of melatonin metabolism and provides insights into healthy living. |
| 1099 |
MPP+로 유도된 파킨슨병 세포 모델에서 Hepad S9-1의 신경세포 보호 효과 |
목서희,박병준, 주인환, 박종민, 김동희 |
20230825 ~ |
학회지 |
| arkinson's disease is the second most common neurodegenerative disease. Levodopa has a good effect for a period of two to five years, but long-term use reduces the effectiveness of the drug and accompanies side effects. To date, there is no strategy that has been able of fundamental treatment of Parkinson's disease. We developed Hepad S9-1 composed of 5 herbal materials (Paeonia lactiflora Pallas, Uncaria sinensis Havil, Spatholobus suberectus Dunn, Panax ginseng C. A. Meyer, and Glycyrrhiza uralensis Fischer). This in vitro study was conducted to search a novel disease-modifying drug, observing the effect of Hepad S9-1, a plant derived compounds on the apoptotic process of PC12 cells which was induced by 1-methyl-4-phenylpyridinium (MPP+). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine the effect of MMP+ or Hepad S9-1 on cell viability in PC12 cells. In addition, we examined cell protective effect of Hepad S9-1 in MPP+-induced PC12 cells. And gene and protein expression level of various factors that regulate apoptosis were confirmed by real-time PCR and western blot analysis. As a result of the study, Hepad S9-1 significantly inhibited MPP+-induced neuronal cell death. Also, Hepad S9-1 inhibited apoptosis-inducing Bcl-2 associated X (Bax) gene and protein expression, cytochrome C protein expression, caspase-9, caspase-7, caspase-3 and poly ADP-ribose polymerase (PARP) activation. In addition, the neuroprotective ability was objectively confirmed by up-regulating the genes and proteins expression of B-cell lymphoma-2 (Bcl-2) and B-cell lymphoma-extra large (Bcl-xl) that defend at apoptosis. Therefore, it seems that Hepad S9-1 can be additionally applied to the treatment of Parkinson's disease to suppress the progression of dopaminergic neuronal cell death. |
| 1098 |
Therapeutic Potential of Silymarin in Inhibiting the Fibroblast-to-Myofibroblast Transition in Renal Interstitial Fibroblasts |
Ha-Ram Kang,Su-Bin Lim, Cho-Long Kim, Jung-Soon Mo, Su Jung Park, Han-Sol Jeong |
20230825 ~ |
학회지 |
| Renal fibrosis (RF) is a prominent pathological feature of chronic kidney disease (CKD), characterized by excessive accumulation of extracellular matrix components, resulting in progressive renal function loss. The fibroblast-to-myofibroblast transition (FMT) plays a pivotal role in renal fibrosis pathogenesis, driving aberrant deposition of extracellular matrix proteins and disruption of tissue architecture. Targeting FMT has emerged as a promising strategy to combat renal fibrosis and preserve kidney function. Silymarin, a flavonoid extract derived from Silybum marianum seeds, has gained attention for its therapeutic potential, particularly in liver diseases, due to its potent antioxidant and anti-inflammatory properties. However, the precise mechanisms underlying its effects on FMT remain unclear. This study aimed to investigate the therapeutic potential of silymarin in inhibiting FMT in NRK-49F renal interstitial fibroblasts. Transforming growth factor-beta 1(TGF-β1) plays a crucial role in promoting FMT through the activation of intracellular signaling pathways and induction of key fibrotic markers, including alpha-smooth muscle actin (α-SMA) and vimentin. Silymarin demonstrated significant downregulation of FMT markers, including α-SMA and vimentin, in TGF-β1-stimulated NRK-49F cells. Our findings highlight silymarin as a promising therapeutic candidate for mitigating renal fibrosis and managing CKD. |
