회원학회

회원학회

번호 연구제목 연구자 연구기간 발표실적
내용
102 작약이 HaCaT 세포에서 아토피 피부염 관련 염증 억제에 미치는 영향 이혜인, 김엄지, 손동빈, 주병덕, 손영주, 김은영, 정혁상 2022-01 ~ 학회지
Objectives : Paeonia lactiflora Pallas (PLP) have been reported to have pharmacological effects such as anti-inflammatory and analgesic. However, it is not yet known whether PLP extract has anti-inflammatory effect on HaCaT cells, human keratinocyte.
Methods : To confirm the anti-inflammatory effect of PLP on keratinocyte, TNF-α/IFN-γ-stimulated HaCaT cells were used. HaCaT cells were pre-treated with PLP for 1h before stimulation with TNF-α/IFN-γ. Then HaCaT cells were stimulated with TNF-α/ IFN-γ for 24 h, the cells and media were harvested to measure the inflammatory cytokines levels. Granulocyte-macrophage colony stimulating factor (GM-CSF), monocyte chemoattractant protein-1 (MCP-1), interleukin 1 beta (IL-1β), and TNF-α were analyzed by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression of thymus and activation-regulated chemokines (TARC), IL-6, and IL-8 were measured by reverse transcription-polymerase chain reaction (RT-PCR). We also investigated the inhibitory mechanism of the mitogen-activated protein kinase (MAPKs) including ERK, JNK, and p38 and nuclear factor-kappaB (NF-κB) by PLP using western blot. Results : PLP did not show cytotoxicity in HaCaT cells. In TNF-α/IFN-γ -stimulated HaCaT cells, PLP significantly inhibited the expression of GM-CSF, MCP-1 IL-1β, TNF-α, TARC and IL-6. PLP inhibited the phosphorylation of ERK and translocation of NF-κB into the nucleus.
Conclusions : These results indicate that PLP
could ameliorate the TNF-α/IFN-γ-stimulated inflammatory response through inhibition of MAPK and NF-kB signal pathway. This suggests that PLP could be used beneficial agent to improve skin inflammation.

Key words : Paeonia Lactiflora Pallas, HaCaT, pro-inflammatory cytokine, atopic dermatitis, anti-inflammatory
101 Sprague-Dawley 랫드를 이용한 소핵시험을 통한 SU어혈약침의 안전성 평가 구자승, 정철, 황지혜 2022-01 ~ 학회지
Objectives : The purpose of this study was to evaluate the potential of the test substance, SU-Eohyeol Pharmacopuncture (SUEP), to induce micronuclei in bone marrow cells of Sprague-Dawley (SD) Rats.
Methods : The dose range preliminary study was performed first. 1 ml/animal was selected as the high dose of this study. Two additional lower dose levels (0.5 and 0.25 ml/animal) were produced by applying a geometric ratio of 2. In addition, the positive and negative control groups were set. Then, after intramuscular administration (1 ml/animal) of SUEP to 8-week-old male SD rats, an in vivo micronucleus test was performed to evaluate the induction of micronuclei in SD rat bone marrow cells.
Results : As a result of the main study, the incidence of micronucleated polychromatic erythrocytes (MNPCE) in polychromatic erythrocytes (PCE) in the test substance SUEP groups was not statistically significantly different from the negative control group. In addition, the ratio of PCE to total erythrocytes in the test substance SUEP groups was not statistically significantly different from the negative control group. In the positive control group, the incidence of MNPCE in PCE was statistically significantly increased when compared to the negative control group. The ratio of PCE to total erythrocytes in the positive control group was not statistically significantly different from the negative control
group.
Conclusions : Based on these results, the test substance, SUEP, did not have any potential to induce micronuclei formation in bone marrow cells of rats under the conditions of this study.

Key words : micronucleus test, Cervi Parvum Cornu, SU-Eohyeol, Jungsongouhyul pharmacopuncture, Immuno-Pharmacopuncture,
toxicity test
100 오수혈 자침 깊이와 맥기의 천심 이서영, 이인선, 채윤병 2022-01 ~ 학회지
Objectives : The purpose of this study was to investigate the needling depth of five-phase acupoints and discuss the association with the depth of meridian qi.
Methods : DongUiBoGam was used to determine the depth of five-phase acupoints. The depth of needling at 60 five-phase acupoints was compared between well, spring, stream, river, and sea acupoints.
Results : The proximal part of the extremities had deeper needling depth than the distal part of the extremities. The targeted deqi sensation can be related to the needling depth. Conclusions : The depth of the meridian qi is related to the distinct patterns of needling depth of five-phase acupoints.

Key words : acupuncture, depth, five-phase acupoints, meridian qi
99 아시혈의 의미와 특성의 이해 이서영, 류연희, 이인선, 채윤병 2022-01 ~ 학회지
Objectives : Acupoints are divided into three categories: classical acupoints, extra-acupoints, and Ashi points. The aim of this study was to understand the meaning and features of Ashi points.
Methods : We examined the original meaning of Ashi points from the classical medical texts, including the Beijiqianjinyaofang, the Essential Prescriptions Worth a Thousand Gold for Emergencies, and the Huangdi Neijing, the Yellow Emperor’s Canon of Internal Medicine.
Results : First, the Ashi method is to locate the points for the acupuncture and moxibustion based on the patients’ reactions mainly manifested by sensations of comfort and pain, which can help identify not only Ashi points but also classical and extra-acupoints. Thus, Ashi points may or may not match to classical or extra-acupoints, and we propose that Ashi points should not be classified mutually exclusive to classical or extra-acupoints. Second, there are several similarities between Ashi points and myofascial trigger points. They are located by palpation and have no fixed anatomical positions. Patients experience painful but pleasant feeling when Ashi and myofascial trigger points are pressed, and stimulation of these points have treatment effects.
Conclusions : We suggest that Ashi method be used to identify the acupoints based on how patients react when these points are pressed. Ashi points may or may not correspond to classical or extra-acupoints, and share traits with myofascial trigger points.

Key words : Ashi points, definition, location, palpation, myofascial trigger points
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