회원학회

회원학회

번호 연구제목 연구자 연구기간 발표실적
내용
514 Journal of Pharmacouncture (Vol 27, No.4) Yujin Choi 2024-12-31 ~ 학회지
Effect of Herbal Medicine (Hwanglyeonhaedok-tang) on Insomnia Patients with Bedtime Procrastination: study protocol for a randomized controlled trial

Objectives: Insomnia, characterized by difficulty initiating or maintaining sleep, affects a
significant portion of the global population. Bedtime procrastination, which is a voluntary
delay in going to bed, is a major contributing factor to the prevalence of sleep deprivation
in modern societies. Hwanglyeonhaedok-tang (HJD decoction) has shown promise in
improving the symptoms of insomnia. This study aimed to evaluate the efficacy of HJD decoction
in patients with insomnia and bedtime procrastination.
Methods: This study employs a parallel-group design, comparing HJD decoction to usual
care in a 1:1 ratio. Sixty participants with insomnia and bedtime procrastination will be enrolled.
The experimental group will receive HJD decoction for 4 weeks, while both groups
will undergo a brief behavioral treatment for insomnia. The primary outcome will be the
change in the Insomnia Severity Index score (ISI) from baseline to 4 weeks. Secondary outcomes
include sleep diary metrics, the Pittsburgh Sleep Quality Index (PSQI), and Bedtime
Procrastination Scale (BPS). The exploratory outcomes include perceived stress, anxiety,
smartphone use, and heart rate variability.
Conclusion: This trial examines the role of herbal medicine in treating a specific type of insomnia
that is increasingly common in modern society. The combination of HJD decoction
with behavioral intervention offers a comprehensive approach to treating insomnia complicated
by bedtime procrastination. The results will provide valuable insights into integrative
treatment strategies for sleep disorders in the digital age.
513 Journal of Pharmacouncture (Vol 27, No.4) Ju-Hee Park 2024-12-31 ~ 학회지
Cannabigerol Treatment Shows Antiproliferative Activity and Causes Apoptosis of Human Colorectal Cancer Cells

Objectives: To determine growth inhibitory and anti-cancer effects of Cannabigerol (CBG)
in human colorectal cancer cells.
Methods: Anti-proliferative effect of CBG was examined using MTT assay and two colorectal
cancer cells (SW480 and LoVo cells). Cell death ratio was analyzed using Annexin V/
PI staining experiment. Cell cycle distribution was analyzed using flow cytometry. We also
performed western blot analysis on apoptotic marker proteins.
Results: CBG showed growth inhibitory effect in colorectal cancer cells using MTT assay.
IC50 concentration of CBG was 34.89 μM in SW480 cells and 23.51 μM in LoVo cells. Annexin
V/PI staining showed that CBG treatment increased apoptotic cells from 4.8% to
31.7% in SW480 cells and from 7.7% to 33.9% in LoVo cells. Flow cytometry confirmed
that CBG increased sub G1 population via G1 arrest in both SW480 and LoVo cells. Western
blot analysis showed that CBG increased expression levels of cell death-related proteins
such as cleaved PARP-1, cleaved caspase 9, p53, and caspase 3.
Conclusion: CBG treatment shows antiproliferative activity and causes apoptosis of
colorectal cancer cells, suggesting that CBG is applicable as a promising anticancer drug.
512 Journal of Pharmacouncture (Vol 27, No.4) Mustakin Ahmed Shohel 2024-12-31 ~ 학회지
Anthelmintic Potential of Discarded Litchi chinensis Seeds: a sustainable approach to agricultural by-product utilization

Objectives: Litchi chinensis (Sonn.), belonging to the Sapindaceae family, has historically
been used for the treatment of gastrointestinal ailments, including ulcers, gastritis,
diarrhea, and infections. Plants in the Sapindaceae family have demonstrated potential
anthelmintic effects, while the efficacy of L. chinensis remains barely investigated. L.
chinensis seeds are often discarded as waste; however, utilizing these seeds promotes
sustainable practices and may provide a natural alternative to conventional anthelmintics.
The study aims to investigate the qualitative phytochemicals and evaluate the anthelmintic
efficacy of L. chinensis seed ethanolic extract (LCSE).
Methods: Fresh L. chinensis fruits were collected from a local market, peeled fruits and removed
aril from the seeds and then washed, air-dried and extracted with ethanol. A qualitative
phytochemical screening and assessment of the anthelmintic properties of LCSE
were conducted using standard procedures. The time required for paralysis and death of
adult earthworms (Eisenia fetida ) was assessed by analyzing each test solution at five distinct
dosages (5, 25, 50, 75, and 100 mg/mL). Albendazole served as the standard, while
distilled water functioned as the control. Statistical analysis was performed using two-way
ANOVA followed by Tukey’s multiple comparison test in GraphPad Prism version 10.1.2.
Results: Qualitative analysis revealed that LCSE is rich in phytochemicals, including alkaloids,
anthraquinones, carbohydrates, flavonoid, glycosides, proteins and amino acids,
phenols, terpenoids, and tannins. The anthelmintic activities of the LCSE and albendazole
were dose-dependent, as the time required for paralysis and mortality decreased in proportion
to the concentrations increased. LCSE exhibited strong anthelmintic efficacy at a
concentration of 100 mg/mL (paralysis: 12.67 ± 0.33, death: 14 ± 0.58), which was significantly
greater than that of albendazole (paralysis: 15 ± 1.15, death: 17.67 ± 1.15).
Conclusion: This finding suggests that LCSE holds potent anthelmintic properties, making
it a promising natural alternative to conventional treatments like albendazole.
511 Journal of Pharmacouncture (Vol 27, No.4) Yunna Kim 2024-12-31 ~ 학회지
Efficacy of Sophora japonica L. Pill in Subjective Memory Complaints in Healthy Adults: a protocol of randomized, double-blind, placebo-controlled, parallel-group, clinical trial

Objectives: Subjective memory complaints, increasingly common among older adults,
may indicate early cognitive decline or dementia. Sophora japonica L. , a herbal medicine
in Korean medicine, has shown potential cognitive benefits in preclinical studies through
neuroprotective and anti-inflammatory properties. Given limited efficacy of current pharmacological
treatments for cognitive impairment and growing interest in natural products,
investigating S. japonica extract in humans is warranted.
Methods: This randomized, double-blind, placebo-controlled trial will involve 100 participants
aged 40-70 years with subjective memory complaints but without diagnosed cognitive
impairment. Participants will receive either S. japonica extract (1,250 mg) or placebo
daily for 8 weeks. The primary outcome is change in digit span test score, assessing shortterm
memory and attention. Secondary outcomes include changes in other cognitive function
tests (visual short-term memory, visual learning, and verbal learning), as well as measures
of depression (Beck Depression Inventory-II), anxiety (State-Trait Anxiety Inventory-
Y), stress (Stress Response Inventory), heart rate variability, and quality of life (EuroQol
5-Dimension-3L). Assessments will be conducted at baseline and after 8 weeks, with
safety monitoring throughout the study period.
Conclusion: This study will provide evidence on the efficacy and safety of S. japonica extract
for improving cognitive function in adults with subjective memory complaints. If proven
effective, this supplement could offer a new approach for supporting cognitive health in
aging populations. The comprehensive assessment of cognitive, mood, and quality of life
outcomes will allow thorough evaluation of its potential benefits.
목록